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General Microbiology Testing

Biological Indicator Testing - BI Testing

The robust and repeatable biological indicator testing procedures utilised at Wickham Micro are aimed at confining the certified population of spores on manufacturer’s biological indicators (BIs) using an extraction, heat shock and plate count method.  Our techniques have been developed over many years of working with end users and BI manufacturers alike.

At Wickham Micro, we understand the critical importance of accurate and reliable Biological Indicator (BI) testing. That's why we've developed robust and repeatable testing procedures, honed through years of collaboration with end-users and BI manufacturers. Our techniques – extraction, heat shock, and meticulous plate count methods – ensure the certified population of spores on your BIs is precisely verified.

Comprehensive Expertise, Rigorous Standards

Our testing protocols strictly adhere to the ISO 11138 series for the sterilisation of healthcare products. We cover all key sterilization and decontamination processes, including:

  • Moist heat
  • Dry heat
  • Ethylene oxide sterilization
  • Low-temperature steam
  • Hydrogen peroxide vapor decontamination

We utilise BIs with standardised bacterial spores (typically a 6-log population of Geobacillus stearothermophilus and Bacillus atrophaeus), precisely matched to the sterilisation method being monitored.

Beyond Standard Testing: D-Value Analysis and Customisation

Wickham Micro offers advanced D-value testing to determine the resistance of microorganisms to specific sterilisation methods. This information is essential for validating and optimising your sterilisation processes.

We test a wide variety of BI formats – paper strips, stainless steel discs, self-contained BIs – and welcome customisation. Our technicians have expertise in bespoke BI production and enumeration projects to support proof-of-concept work, research publications, and manufacturer claims.

Complete Solutions for Your BI Testing Needs

In addition to expert testing, Wickham Micro offers:

  • BI troubleshooting
  • BI and media procurement assistance
  • Validation and commissioning studies, especially for autoclaves
  • Rigorous pre-testing enumeration of all BIs

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Wickham Micro: Your Partner in Sterilisation Assurance

Contact us today to discuss your Biological Indicator testing needs.

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